Research
Research lines
- Characterization of molecular and cellular mechanisms involved in the development of cardiovascular damage associated to obesity
- Characterization of molecular and cellular mechanisms involved in the development of cardiovascular damage associated to chronic kidney disease
- Biomarkers of oxidative stress in cardiovascular disease
- Role of perivascular adipose tissue (PVAT) on vascular function
- Hormonal regulation of energy metabolism, food intake and behavior in models of obesity
- Study of natural products, drugs and diets useful in the treatment and prevention of cardiovascular damage
- Translational research in obesity and chronic kidney disease
- Impact of pharmaceutical care and pharmacist intervention on reducing cardiovascular risk in obese patients
Animal models
- obesity (genetic models; high fat diet (HFD)-induced obesity
- caloric restriction
- hypertension
- chronic kidney disease
- diabetic nephropathy
"In vivo" methods in rats and mice
- Blood Pressure and ECG
- Intraventricular pressure
- Cardiac and kidney function by NMR
- Adipose tissue distribution and metabolism
- Pharmacological treatments
"in vitro" techniques
- Large artery function by isometric tension recording
- Mesenteric bed function by perfusion pressure recording
- Wire myography
- Laser scanning confocal microscopy
- Cell culture: human and murine vascular smooth muscle cells, vascular fibroblasts, endothelial cells, mesenchymal stem cells
- Oxidative stress parameter determination
- Molecular determinations: protein expression (Western blot) and mRNA (RT-qPCR), immunoprecipitation
- Biochemical studies: activity of metalloproteinases, renin-angiotensin system enymes, AMPK activity
Role of perivascular adipose tissue (PVAT) on vascular structure and function
