GRUPO DE INVESTIGACIÓN

	Jesús Pérez Gil Catedrático de Universidad jperezgil@ucm.es
	Olga Cañadas Benito Profesora Contratada Doctor ocanadas@quim.ucm.es
	Antonio Cruz Rodríguez Profesor Contratado Doctor acruz@ucm.es
	Mercedes Echaide Torreguitar Profesora Contratada Doctor mechaide@ucm.es
	Begoña García Álvarez Profesora Contratada Doctor begoga01@ucm.es
	Lucia García Ortega Profesora Contratada Doctor luciagar@pdi.ucm.es
	Bárbara Olmeda Lozano Profesora Contratada Doctor bolmeda@bio.ucm.es
	Alejandro Alonso Eugenio Becario Predoctoral alejaalo@ucm.es
	Ainhoa Collada Marugán Contratada Predoctoral ainhoaco@ucm.es
	Cristina García Mouton Becaria Predoctoral crigar12@ucm.es
	Miriam Isasi Campillo Becaria Predoctoral misasi@ucm.es
	Michelle Morán Lalangui Investigadora Contratada Predoctoral jumoran@ucm.es
	Amaya Blanco Rivero Investigadora Contratada Predoctoral amayabla@ucm.es
	Nuria Puado Villa Personal Administrativo npuado@ucm.es

PUBLICACIONES

[1] Castillo-Sanchez, J. C., Roldan, N., Garcia-Alvarez, B., Batllori, E., Galindo, A., Cruz, A., and Perez-Gil, J. (2021) The highly packed and dehydrated structure of pre-formed unexposed human pulmonary surfactant isolated from amniotic fluid, Am J Physiol Lung Cell Mol Physiol.

[2] Testoni, G., Olmeda, B., Duran, J., Lopez-Rodriguez, E., Aguilera, M., Hernandez-Alvarez, M. I., Prats, N., Perez-Gil, J., and Guinovart, J. J. (2021) Pulmonary glycogen deficiency as a new potential cause of respiratory distress syndrome, Hum Mol Genet 29, 3554-3565.

[3] Schipke, J., Jutte, D., Brandenberger, C., Autilio, C., Perez-Gil, J., Bernhard, W., Ochs, M., and Muhlfeld, C. (2021) Dietary Carbohydrates and Fat Induce Distinct Surfactant Alterations in Mice, Am J Respir Cell Mol Biol 64, 379-390.

[4] Paget, T. L., Parkinson-Lawrence, E. J., Trim, P. J., Autilio, C., Panchal, M. H., Koster, G., Echaide, M., Snel, M. F., Postle, A. D., Morrison, J. L., Perez-Gil, J., and Orgeig, S. (2021) Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse, Cells 10.

[5] Martinez-Calle, M., Parra-Ortiz, E., Cruz, A., Olmeda, B., and Perez-Gil, J. (2021) Towards the Molecular Mechanism of Pulmonary Surfactant Protein SP-B: At the Crossroad of Membrane Permeability and Interfacial Lipid Transfer, J Mol Biol 433, 166749.

[6] Hidalgo, A., Garcia-Mouton, C., Autilio, C., Carravilla, P., Orellana, G., Islam, M. N., Bhattacharya, J., Bhattacharya, S., Cruz, A., and Perez-Gil, J. (2021) Pulmonary surfactant and drug delivery: Vehiculization, release and targeting of surfactant/tacrolimus formulations, J Control Release 329, 205-222.

[7] Guagliardo, R., Herman, L., Penders, J., Zamborlin, A., De Keersmaecker, H., Van de Vyver, T., Verstraeten, S., Merckx, P., Mingeot-Leclercq, M. P., Echaide, M., Perez-Gil, J., Stevens, M. M., De Smedt, S. C., and Raemdonck, K. (2021) Surfactant Protein B Promotes Cytosolic SiRNA Delivery by Adopting a Virus-like Mechanism of Action, ACS Nano 15, 8095-8109.

[8] De Luca, D., Cogo, P., Kneyber, M. C., Biban, P., Semple, M. G., Perez-Gil, J., Conti, G., Tissieres, P., and Rimensberger, P. C. (2021) Surfactant therapies for pediatric and neonatal ARDS: ESPNIC expert consensus opinion for future research steps, Crit Care 25, 75.

[9] Da Silva, E., Vogel, U., Hougaard, K. S., Perez-Gil, J., Zuo, Y. Y., and Sorli, J. B. (2021) An adverse outcome pathway for lung surfactant function inhibition leading to decreased lung function, Curr Res Toxicol 2, 225-236.

[10] Da Silva, E., Autilio, C., Hougaard, K. S., Baun, A., Cruz, A., Perez-Gil, J., and Sorli, J. B. (2021) Molecular and biophysical basis for the disruption of lung surfactant function by chemicals, Biochim Biophys Acta Biomembr 1863, 183499.

[11] Castillo-Sanchez, J. C., Cruz, A., and Perez-Gil, J. (2021) Structural hallmarks of lung surfactant: Lipid-protein interactions, membrane structure and future challenges, Arch Biochem Biophys 703, 108850.

[12] Castillo-Sanchez, J. C., Cerrada, A., Conde, M., Cruz, A., and Perez-Gil, J. (2021) Compositional, structural and functional properties of discrete coexisting complexes within bronchoalveolar pulmonary surfactant, Biochim Biophys Acta Biomembr 1864, 183808.

[13] Canadas, O., Garcia-Garcia, A., Prieto, M. A., and Perez-Gil, J. (2021) Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant, Nanomaterials (Basel) 11.

[14] Barriga, A., Moran-Lalangui, M., Castillo-Sanchez, J. C., Mingarro, I., Perez-Gil, J., and Garcia-Alvarez, B. (2021) Role of pulmonary surfactant protein Sp-C dimerization on membrane fragmentation: An emergent mechanism involved in lung defense and homeostasis, Biochim Biophys Acta Biomembr 1863, 183572.

[15] Autilio, C., Echaide, M., Cruz, A., Garcia-Mouton, C., Hidalgo, A., Da Silva, E., De Luca, D., Sorli, J. B., and Perez-Gil, J. (2021) Molecular and biophysical mechanisms behind the enhancement of lung surfactant function during controlled therapeutic hypothermia, Sci Rep 11, 728.

[16] Yazicioglu, T., Muhlfeld, C., Autilio, C., Huang, C. K., Bar, C., Dittrich-Breiholz, O., Thum, T., Perez-Gil, J., Schmiedl, A., and Brandenberger, C. (2020) Aging impairs alveolar epithelial type II cell function in acute lung injury, Am J Physiol Lung Cell Mol Physiol 319, L755-L769.

[17] Sorli, J. B., Lag, M., Ekeren, L., Perez-Gil, J., Haug, L. S., Da Silva, E., Matrod, M. N., Gutzkow, K. B., and Lindeman, B. (2020) Per- and polyfluoroalkyl substances (PFASs) modify lung surfactant function and pro-inflammatory responses in human bronchial epithelial cells, Toxicol In Vitro 62, 104656.

[18] Ruwisch, J., Sehlmeyer, K., Roldan, N., Garcia-Alvarez, B., Perez-Gil, J., Weaver, T. E., Ochs, M., Knudsen, L., and Lopez-Rodriguez, E. (2020) Air Space Distension Precedes Spontaneous Fibrotic Remodeling and Impaired Cholesterol Metabolism in the Absence of Surfactant Protein C, Am J Respir Cell Mol Biol 62, 466-478.

[19] Pineiro-Hermida, S., Autilio, C., Martinez, P., Bosch, F., Perez-Gil, J., and Blasco, M. A. (2020) Telomerase treatment prevents lung profibrotic pathologies associated with physiological aging, J Cell Biol 219.

[20] Oeo-Santos, C., Lopez-Rodriguez, J. C., Garcia-Mouton, C., San Segundo-Acosta, P., Jurado, A., Moreno-Aguilar, C., Garcia-Alvarez, B., Perez-Gil, J., Villalba, M., Barderas, R., and Cruz, A. (2020) Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids, Biochim Biophys Acta Biomembr 1862, 183258.

[21] Mato, A., Blanco, F. G., Maestro, B., Sanz, J. M., Perez-Gil, J., and Prieto, M. A. (2020) DISSECTING THE POLYHYDROXYALKANOATE-BINDING DOMAIN OF THE PhaF PHASIN: RATIONAL DESIGN OF A MINIMIZED AFFINITY TAG, Appl Environ Microbiol.

[22] Martinez-Calle, M., Prieto, M., Olmeda, B., Fedorov, A., Loura, L. M. S., and Perez-Gil, J. (2020) Pulmonary surfactant protein SP-B nanorings induce the multilamellar organization of surfactant complexes, Biochim Biophys Acta Biomembr 1862, 183216.

[23] Liekkinen, J., Enkavi, G., Javanainen, M., Olmeda, B., Perez-Gil, J., and Vattulainen, I. (2020) Pulmonary Surfactant Lipid Reorganization Induced by the Adsorption of the Oligomeric Surfactant Protein B Complex, J Mol Biol.

[24] Garcia-Mouton, C., Hidalgo, A., Arroyo, R., Echaide, M., Cruz, A., and Perez-Gil, J. (2020) Pulmonary Surfactant and Drug Delivery: An Interface-Assisted Carrier to Deliver Surfactant Protein SP-D Into the Airways, Front Bioeng Biotechnol 8, 613276.

[25] Echaide, M., Autilio, C., Lopez-Rodriguez, E., Cruz, A., and Perez-Gil, J. (2020) In Vitro Functional and Structural Characterization of A Synthetic Clinical Pulmonary Surfactant with Enhanced Resistance to Inhibition, Sci Rep 10, 1385.

[26] De Luca, D., Shankar-Aguilera, S., Autilio, C., Raschetti, R., Vedovelli, L., Fitting, C., Payre, C., Jeammet, L., Perez-Gil, J., Cogo, P. E., Carnielli, V. P., Lambeau, G., and Touqui, L. (2020) Surfactant-secreted phospholipase A2 interplay and respiratory outcome in preterm neonates, Am J Physiol Lung Cell Mol Physiol 319, L95-L104.

[27] Canadas, O., Olmeda, B., Alonso, A., and Perez-Gil, J. (2020) Lipid-Protein and Protein-Protein Interactions in the Pulmonary Surfactant System and Their Role in Lung Homeostasis, Int J Mol Sci 21.

[28] Autilio, C., Echaide, M., Shankar-Aguilera, S., Bragado, R., Amidani, D., Salomone, F., Perez-Gil, J., and De Luca, D. (2020) Surfactant Injury in the Early Phase of Severe Meconium Aspiration Syndrome, Am J Respir Cell Mol Biol 63, 327-337.

[29] Autilio, C., Echaide, M., Dell'Orto, V., Perez-Gil, J., and De Luca, D. (2020) Effect of Whole Body Hypothermia on Surfactant Function When Amniotic Fluid Is Meconium Stained, Ther Hypothermia Temp Manag 10, 186-189.

[30] Arroyo, R., Echaide, M., Wilmanowski, R., Martin-Gonzalez, A., Batllori, E., Galindo, A., Rosenbaum, J. S., Moreno-Herrero, F., Kingma, P. S., and Perez-Gil, J. (2020) Structure and activity of human surfactant protein D from different natural sources, Am J Physiol Lung Cell Mol Physiol 319, L148-L158.

[31] Arroyo, R., Echaide, M., Moreno-Herrero, F., Perez-Gil, J., and Kingma, P. S. (2020) Functional characterization of the different oligomeric forms of human surfactant protein SP-D, Biochim Biophys Acta Proteins Proteom 1868, 140436.

[32] Mato, A., Tarazona, N. A., Hidalgo, A., Cruz, A., Jimenez, M., Perez-Gil, J., and Prieto, M. A. (2019) Interfacial Activity of Phasin PhaF from Pseudomonas putida KT2440 at Hydrophobic-Hydrophilic Biointerfaces, Langmuir : the ACS journal of surfaces and colloids 35, 678-686.

[33] Martinez-Calle, M., Alonso, A., Perez-Gil, J., and Olmeda, B. (2019) Native supramolecular protein complexes in pulmonary surfactant: Evidences for SP-A/SP-B interactions, J Proteomics 207, 103466.

[34] Lopez-Rodriguez, E., Roldan, N., Garcia-Alvarez, B., and Perez-Gil, J. (2019) Protein and lipid fingerprinting of native-like membrane complexes by combining TLC and protein electrophoresis, Journal of lipid research 60, 430-435.

[35] Garcia-Mouton, C., Hidalgo, A., Cruz, A., and Perez-Gil, J. (2019) The Lord of the Lungs: The essential role of pulmonary surfactant upon inhalation of nanoparticles, Eur J Pharm Biopharm 144, 230-243.

[36] Autilio, C., and Perez-Gil, J. (2019) Understanding the principle biophysics concepts of pulmonary surfactant in health and disease, Arch Dis Child Fetal Neonatal Ed 104, F443-F451.

[37] Arroyo, R., Khan, M. A., Echaide, M., Perez-Gil, J., and Palaniyar, N. (2019) SP-D attenuates LPS-induced formation of human neutrophil extracellular traps (NETs), protecting pulmonary surfactant inactivation by NETs, Commun Biol 2, 470.

[38] Merckx, P., De Backer, L., Van Hoecke, L., Guagliardo, R., Echaide, M., Baatsen, P., Olmeda, B., Saelens, X., Perez-Gil, J., De Smedt, S. C., and Raemdonck, K. (2018) Surfactant protein B (SP-B) enhances the cellular siRNA delivery of proteolipid coated nanogels for inhalation therapy, Acta Biomater 78, 236-246.

[39] Martinez-Calle, M., Olmeda, B., Dietl, P., Frick, M., and Perez-Gil, J. (2018) Pulmonary surfactant protein SP-B promotes exocytosis of lamellar bodies in alveolar type II cells, FASEB J 32, 4600-4611.

[40] Madsen, J., Panchal, M. H., Mackay, R. A., Echaide, M., Koster, G., Aquino, G., Pelizzi, N., Perez-Gil, J., Salomone, F., Clark, H. W., and Postle, A. D. (2018) Metabolism of a synthetic compared with a natural therapeutic pulmonary surfactant in adult mice, Journal of lipid research 59, 1880-1892.

[41] Lugones, Y., Blanco, O., Lopez-Rodriguez, E., Echaide, M., Cruz, A., and Perez-Gil, J. (2018) Inhibition and counterinhibition of Surfacen, a clinical lung surfactant of natural origin, PloS one 13, e0204050.

[42] Guagliardo, R., Perez-Gil, J., De Smedt, S., and Raemdonck, K. (2018) Pulmonary surfactant and drug delivery: Focusing on the role of surfactant proteins, J Control Release 291, 116-126.

[43] Cabre, E. J., Martinez-Calle, M., Prieto, M., Fedorov, A., Olmeda, B., Loura, L. M. S., and Perez-Gil, J. (2018) Homo- and hetero-oligomerization of hydrophobic pulmonary surfactant proteins SP-B and SP-C in surfactant phospholipid membranes, The Journal of biological chemistry 293, 9399-9411.

[44] Autilio, C., Echaide, M., De Luca, D., and Perez-Gil, J. (2018) Controlled hypothermia may improve surfactant function in asphyxiated neonates with or without meconium aspiration syndrome, PloS one 13, e0192295.

[45] Arroyo, R., Martin-Gonzalez, A., Echaide, M., Jain, A., Brondyk, W. H., Rosenbaum, J., Moreno-Herrero, F., and Perez-Gil, J. (2018) Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D, J Mol Biol 430, 1495-1509.

[46] Roldan, N., Perez-Gil, J., Morrow, M. R., and Garcia-Alvarez, B. (2017) Divide & Conquer: Surfactant Protein SP-C and Cholesterol Modulate Phase Segregation in Lung Surfactant, Biophysical journal 113, 847-859.

[47] Olmeda, B., Martinez-Calle, M., and Perez-Gil, J. (2017) Pulmonary surfactant metabolism in the alveolar airspace: Biogenesis, extracellular conversions, recycling, Ann Anat 209, 78-92.

[48] Lemke, A., Castillo-Sanchez, J. C., Prodinger, F., Ceranic, A., Hennerbichler-Lugscheider, S., Perez-Gil, J., Redl, H., and Wolbank, S. (2017) Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant, Sci Rep 7, 6406.

[49] Hidalgo, A., Salomone, F., Fresno, N., Orellana, G., Cruz, A., and Perez-Gil, J. (2017) Efficient Interfacially Driven Vehiculization of Corticosteroids by Pulmonary Surfactant, Langmuir : the ACS journal of surfaces and colloids 33, 7929-7939.

[50] Hidalgo, A., Cruz, A., and Perez-Gil, J. (2017) Pulmonary surfactant and nanocarriers: Toxicity versus combined nanomedical applications, Biochim Biophys Acta Biomembr 1859, 1740-1748.

[51] Echaide, M., Autilio, C., Arroyo, R., and Perez-Gil, J. (2017) Restoring pulmonary surfactant membranes and films at the respiratory surface, Biochim Biophys Acta Biomembr 1859, 1725-1739.

[52] Carravilla, P., Cruz, A., Martin-Ugarte, I., Oar-Arteta, I. R., Torralba, J., Apellaniz, B., Perez-Gil, J., Requejo-Isidro, J., Huarte, N., and Nieva, J. L. (2017) Effects of HIV-1 gp41-Derived Virucidal Peptides on Virus-like Lipid Membranes, Biophysical journal 113, 1301-1310.

[53] Autilio, C., Echaide, M., Dell'Orto, V., Perez-Gil, J., and De Luca, D. (2017) Effect of Whole Body Hypothermia on Surfactant Function When Amniotic Fluid is Meconium Stained, Ther Hypothermia Temp Manag.

[54] Autilio, C., Echaide, M., Benachi, A., Marfaing-Koka, A., Capoluongo, E. D., Perez-Gil, J., and De Luca, D. (2017) A Noninvasive Surfactant Adsorption Test Predicting the Need for Surfactant Therapy in Preterm Infants Treated with Continuous Positive Airway Pressure, J Pediatr 182, 66-73 e61.

[55] Roldan, N., Nyholm, T. K. M., Slotte, J. P., Perez-Gil, J., and Garcia-Alvarez, B. (2016) Effect of Lung Surfactant Protein SP-C and SP-C-Promoted Membrane Fragmentation on Cholesterol Dynamics, Biophysical journal 111, 1703-1713.

[56] Lopez-Rodriguez, J. C., Barderas, R., Echaide, M., Perez-Gil, J., Villalba, M., Batanero, E., and Cruz, A. (2016) Surface activity as a crucial factor of the biological actions of Ole e 1, the main aeroallergen of olive tree (Olea europaea) pollen, Langmuir : the ACS journal of surfaces and colloids.

[57] Lopez-Rodriguez, E., Pascual, A., Arroyo, R., Floros, J., and Perez-Gil, J. (2016) Human Pulmonary Surfactant Protein SP-A1 Provides Maximal Efficiency of Lung Interfacial Films, Biophysical journal 111, 524-536.

[58] Lopez-Rodriguez, E., Boden, C., Echaide, M., Perez-Gil, J., Kolb, M., Gauldie, J., Maus, U. A., Ochs, M., and Knudsen, L. (2016) Surfactant dysfunction during overexpression of TGF-beta1 precedes profibrotic lung remodeling in vivo, Am J Physiol Lung Cell Mol Physiol 310, L1260-1271.

[59] Huarte, N., Carravilla, P., Cruz, A., Lorizate, M., Nieto-Garai, J. A., Krausslich, H. G., Perez-Gil, J., Requejo-Isidro, J., and Nieva, J. L. (2016) Functional organization of the HIV lipid envelope, Sci Rep 6, 34190.

[60] Hobi, N., Giolai, M., Olmeda, B., Miklavc, P., Felder, E., Walther, P., Dietl, P., Frick, M., Perez-Gil, J., and Haller, T. (2016) A small key unlocks a heavy door: The essential function of the small hydrophobic proteins SP-B and SP-C to trigger adsorption of pulmonary surfactant lamellar bodies, Biochimica et biophysica acta 1863, 2124-2134.

[61] Banares-Hidalgo, A., Perez-Gil, J., and Estrada, P. (2016) Conformational Stability of the NH2-Terminal Propeptide of the Precursor of Pulmonary Surfactant Protein SP-B, PloS one 11, e0158430.

[62] Roldan, N., Goormaghtigh, E., Perez-Gil, J., and Garcia-Alvarez, B. (2015) Palmitoylation as a key factor to modulate SP-C-lipid interactions in lung surfactant membrane multilayers, Biochimica et biophysica acta 1848, 184-191.

[63] Raesch, S. S., Tenzer, S., Storck, W., Rurainski, A., Selzer, D., Ruge, C. A., Perez-Gil, J., Schaefer, U. F., and Lehr, C. M. (2015) Proteomic and Lipidomic Analysis of Nanoparticle Corona upon Contact with Lung Surfactant Reveals Differences in Protein, but Not Lipid Composition, ACS Nano 9, 11872-11885.

[64] Perez-Gil, J., and Monroy, F. P. (2015) Surfing the continuous and walking amongst molecules to unravel the mechanical properties of biomembranes, Chemistry and physics of lipids 185, 1-2.

[65] Parra, E., and Perez-Gil, J. (2015) Composition, structure and mechanical properties define performance of pulmonary surfactant membranes and films, Chemistry and physics of lipids 185, 153-175.

[66] Olmeda, B., Garcia-Alvarez, B., Gomez, M. J., Martinez-Calle, M., Cruz, A., and Perez-Gil, J. (2015) A model for the structure and mechanism of action of pulmonary surfactant protein B, FASEB J 29, 4236-4247.

[67] Hidalgo, A., Cruz, A., and Perez-Gil, J. (2015) Barrier or carrier? Pulmonary surfactant and drug delivery, Eur J Pharm Biopharm 95, 117-127.

[68] De Backer, L., Cerrada, A., Perez-Gil, J., De Smedt, S. C., and Raemdonck, K. (2015) Bio-inspired materials in drug delivery: Exploring the role of pulmonary surfactant in siRNA inhalation therapy, J Control Release 220, 642-650.

[69] Cerrada, A., Haller, T., Cruz, A., and Perez-Gil, J. (2015) Pneumocytes Assemble Lung Surfactant as Highly Packed/Dehydrated States with Optimal Surface Activity, Biophysical journal 109, 2295-2306.

[70] Olmeda, B., Umstead, T. M., Silveyra, P., Pascual, A., Lopez-Barneo, J., Phelps, D. S., Floros, J., and Perez-Gil, J. (2014) Effect of hypoxia on lung gene expression and proteomic profile: insights into the pulmonary surfactant response, J Proteomics 101, 179-191.

[71] Lopez-Rodriguez, E., and Perez-Gil, J. (2014) Structure-function relationships in pulmonary surfactant membranes: from biophysics to therapy, Biochimica et biophysica acta 1838, 1568-1585.

[72] Hobi, N., Siber, G., Bouzas, V., Ravasio, A., Perez-Gil, J., and Haller, T. (2014) Physiological variables affecting surface film formation by native lamellar body-like pulmonary surfactant particles, Biochimica et biophysica acta 1838, 1842-1850.

[73] De Luca, D., Vazquez-Sanchez, S., Minucci, A., Echaide, M., Piastra, M., Conti, G., Capoluongo, E. D., and Perez-Gil, J. (2014) Effect of whole body hypothermia on inflammation and surfactant function in asphyxiated neonates, Eur Respir J 44, 1708-1710.

[74] Cerrada, A., de la Torre, P., Grande, J., Haller, T., Flores, A. I., and Perez-Gil, J. (2014) Human decidua-derived mesenchymal stem cells differentiate into functional alveolar type II-like cells that synthesize and secrete pulmonary surfactant complexes, PloS one 9, e110195.

[75] Bouzas, V., Haller, T., Hobi, N., Felder, E., Pastoriza-Santos, I., and Perez-Gil, J. (2014) Nontoxic impact of PEG-coated gold nanospheres on functional pulmonary surfactant-secreting alveolar type II cells, Nanotoxicology 8, 813-823.

[76] Banares-Hidalgo, A., Perez-Gil, J., and Estrada, P. (2014) Acidic pH triggers conformational changes at the NH2-terminal propeptide of the precursor of pulmonary surfactant protein B to form a coiled coil structure, Biochimica et biophysica acta 1838, 1738-1751.

[77] Suri, L. N., Cruz, A., Veldhuizen, R. A., Staples, J. F., Possmayer, F., Orgeig, S., and Perez-Gil, J. (2013) Adaptations to hibernation in lung surfactant composition of 13-lined ground squirrels influence surfactant lipid phase segregation properties, Biochimica et biophysica acta 1828, 1707-1714.

[78] Parra, E., Alcaraz, A., Cruz, A., Aguilella, V. M., and Perez-Gil, J. (2013) Hydrophobic pulmonary surfactant proteins SP-B and SP-C induce pore formation in planar lipid membranes: evidence for proteolipid pores, Biophysical journal 104, 146-155.

[79] Olmeda, B., Garcia-Alvarez, B., and Perez-Gil, J. (2013) Structure-function correlations of pulmonary surfactant protein SP-B and the saposin-like family of proteins, European biophysics journal : EBJ 42, 209-222.

[80] Lopez-Rodriguez, E., Cruz, A., Richter, R. P., Taeusch, H. W., and Perez-Gil, J. (2013) Transient exposure of pulmonary surfactant to hyaluronan promotes structural and compositional transformations into a highly active state, The Journal of biological chemistry 288, 29872-29881.

[81] De Luca, D., Vendittelli, F., Trias, J., Fraser, H., Minucci, A., Gentile, L., Perez-Gil, J., Conti, G., Antonelli, M., and Capoluongo, E. D. (2013) Surfactant and Varespladib Co-Administration in Stimulated Rat Alveolar Macrophages Culture, Current pharmaceutical biotechnology.

[82] De Luca, D., Lopez-Rodriguez, E., Minucci, A., Vendittelli, F., Gentile, L., Stival, E., Conti, G., Piastra, M., Antonelli, M., Echaide, M., Perez-Gil, J., and Capoluongo, E. D. (2013) Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants, Crit Care 17, R163.

[83] Bernardino de la Serna, J., Vargas, R., Picardi, V., Cruz, A., Arranz, R., Valpuesta, J. M., Mateu, L., and Perez-Gil, J. (2013) Segregated ordered lipid phases and protein-promoted membrane cohesivity are required for pulmonary surfactant films to stabilize and protect the respiratory surface, Faraday Discuss 161, 535-548; discussion 563-589.

[84] Suri, L. N., McCaig, L., Picardi, M. V., Ospina, O. L., Veldhuizen, R. A., Staples, J. F., Possmayer, F., Yao, L. J., Perez-Gil, J., and Orgeig, S. (2012) Adaptation to low body temperature influences pulmonary surfactant composition thereby increasing fluidity while maintaining appropriately ordered membrane structure and surface activity, Biochimica et biophysica acta 1818, 1581-1589.

[85] Seehase, M., Collins, J. J., Kuypers, E., Jellema, R. K., Ophelders, D. R., Ospina, O. L., Perez-Gil, J., Bianco, F., Garzia, R., Razzetti, R., and Kramer, B. W. (2012) New surfactant with SP-B and C analogs gives survival benefit after inactivation in preterm lambs, PloS one 7, e47631.

[86] Ruge, C. A., Schaefer, U. F., Herrmann, J., Kirch, J., Canadas, O., Echaide, M., Perez-Gil, J., Casals, C., Muller, R., and Lehr, C. M. (2012) The interplay of lung surfactant proteins and lipids assimilates the macrophage clearance of nanoparticles, PloS one 7, e40775.

[87] Lukovic, D., Cruz, A., Gonzalez-Horta, A., Almlen, A., Curstedt, T., Mingarro, I., and Perez-Gil, J. (2012) Interfacial behavior of recombinant forms of human pulmonary surfactant protein SP-C, Langmuir : the ACS journal of surfaces and colloids 28, 7811-7825.

[88] Lopez-Rodriguez, E., Ospina, O. L., Echaide, M., Taeusch, H. W., and Perez-Gil, J. (2012) Exposure to polymers reverses inhibition of pulmonary surfactant by serum, meconium, or cholesterol in the captive bubble surfactometer, Biophysical journal 103, 1451-1459.

[89] Campelo, F., Cruz, A., Perez-Gil, J., Vazquez, L., and Hernandez-Machado, A. (2012) Phase-field model for the morphology of monolayer lipid domains, The European physical journal. E, Soft matter 35, 49.

[90] Cabre, E. J., Loura, L. M., Fedorov, A., Perez-Gil, J., and Prieto, M. (2012) Topology and lipid selectivity of pulmonary surfactant protein SP-B in membranes: Answers from fluorescence, Biochimica et biophysica acta 1818, 1717-1725.

[91] Blanco, O., Cruz, A., Ospina, O. L., Lopez-Rodriguez, E., Vazquez, L., and Perez-Gil, J. (2012) Interfacial behavior and structural properties of a clinical lung surfactant from porcine source, Biochimica et biophysica acta 1818, 2756-2766.

[92] Ruge, C. A., Kirch, J., Canadas, O., Schneider, M., Perez-Gil, J., Schaefer, U. F., Casals, C., and Lehr, C. M. (2011) Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A, Nanomedicine : nanotechnology, biology, and medicine 7, 690-693.

[93] Picardi, M. V., Cruz, A., Orellana, G., and Perez-Gil, J. (2011) Phospholipid packing and hydration in pulmonary surfactant membranes and films as sensed by LAURDAN, Biochimica et biophysica acta 1808, 696-705.

[94] Parra, E., Moleiro, L. H., Lopez-Montero, I., Cruz, A., Monroy, F., and Perez-Gil, J. (2011) A combined action of pulmonary surfactant proteins SP-B and SP-C modulates permeability and dynamics of phospholipid membranes, The Biochemical journal 438, 555-564.

[95] Lu, K. W., Perez-Gil, J., Echaide, M., and Taeusch, H. W. (2011) Pulmonary surfactant proteins and polymer combinations reduce surfactant inhibition by serum, Biochimica et biophysica acta 1808, 2366-2373.

[96] Lopez-Rodriguez, E., Echaide, M., Cruz, A., Taeusch, H. W., and Perez-Gil, J. (2011) Meconium impairs pulmonary surfactant by a combined action of cholesterol and bile acids, Biophysical journal 100, 646-655.

[97] Schurch, D., Ospina, O. L., Cruz, A., and Perez-Gil, J. (2010) Combined and independent action of proteins SP-B and SP-C in the surface behavior and mechanical stability of pulmonary surfactant films, Biophysical journal 99, 3290-3299.

[98] Ravasio, A., Olmeda, B., Bertocchi, C., Haller, T., and Perez-Gil, J. (2010) Lamellar bodies form solid three-dimensional films at the respiratory air-liquid interface, The Journal of biological chemistry 285, 28174-28182.

[99] Pynn, C. J., Picardi, M. V., Nicholson, T., Wistuba, D., Poets, C. F., Schleicher, E., Perez-Gil, J., and Bernhard, W. (2010) Myristate is selectively incorporated into surfactant and decreases dipalmitoylphosphatidylcholine without functional impairment, American journal of physiology. Regulatory, integrative and comparative physiology 299, R1306-1316.

[100] Perez-Gil, J., and Weaver, T. E. (2010) Pulmonary surfactant pathophysiology: current models and open questions, Physiology 25, 132-141.

[101] Olmeda, B., Villen, L., Cruz, A., Orellana, G., and Perez-Gil, J. (2010) Pulmonary surfactant layers accelerate O(2) diffusion through the air-water interface, Biochimica et biophysica acta 1798, 1281-1284.

[102] Baumgart, F., Ospina, O. L., Mingarro, I., Rodriguez-Crespo, I., and Perez-Gil, J. (2010) Palmitoylation of pulmonary surfactant protein SP-C is critical for its functional cooperation with SP-B to sustain compression/expansion dynamics in cholesterol-containing surfactant films, Biophysical journal 99, 3234-3243.

[103] Lu, K. W., Perez-Gil, J., and Taeusch, H. (2009) Kinematic viscosity of therapeutic pulmonary surfactants with added polymers, Biochimica et biophysica acta 1788, 632-637.

[104] Gomez-Gil, L., Schurch, D., Goormaghtigh, E., and Perez-Gil, J. (2009) Pulmonary surfactant protein SP-C counteracts the deleterious effects of cholesterol on the activity of surfactant films under physiologically relevant compression-expansion dynamics, Biophysical journal 97, 2736-2745.

[105] Gomez-Gil, L., Perez-Gil, J., and Goormaghtigh, E. (2009) Cholesterol modulates the exposure and orientation of pulmonary surfactant protein SP-C in model surfactant membranes, Biochimica et biophysica acta 1788, 1907-1915.

[106] Garcia-Verdugo, I., Garcia de Paco, E., Espinassous, Q., Gonzalez-Horta, A., Synguelakis, M., Kanellopoulos, J., Rivas, L., Chaby, R., and Perez-Gil, J. (2009) Synthetic peptides representing the N-terminal segment of surfactant protein C modulate LPS-stimulated TNF-alpha production by macrophages, Innate immunity 15, 53-62.

[107] Cabre, E. J., Malmstrom, J., Sutherland, D., Perez-Gil, J., and Otzen, D. E. (2009) Surfactant protein SP-B strongly modifies surface collapse of phospholipid vesicles: insights from a quartz crystal microbalance with dissipation, Biophysical journal 97, 768-776.

[108] Bernardino de la Serna, J., Oradd, G., Bagatolli, L. A., Simonsen, A. C., Marsh, D., Lindblom, G., and Perez-Gil, J. (2009) Segregated phases in pulmonary surfactant membranes do not show coexistence of lipid populations with differentiated dynamic properties, Biophysical journal 97, 1381-1389.

[109] Ravasio, A., Cruz, A., Perez-Gil, J., and Haller, T. (2008) High-throughput evaluation of pulmonary surfactant adsorption and surface film formation, Journal of lipid research 49, 2479-2488.

[110] Plasencia, I., Baumgart, F., Andreu, D., Marsh, D., and Perez-Gil, J. (2008) Effect of acylation on the interaction of the N-Terminal segment of pulmonary surfactant protein SP-C with phospholipid membranes, Biochimica et biophysica acta 1778, 1274-1282.

[111] Perez-Gil, J. (2008) Properly interpreting lipid-protein specificities in pulmonary surfactant, Biophysical journal 94, 1542-1543; discussion 1544.

[112] Perez-Gil, J. (2008) Structure of pulmonary surfactant membranes and films: the role of proteins and lipid-protein interactions, Biochimica et biophysica acta 1778, 1676-1695.

[113] Mingarro, I., Lukovic, D., Vilar, M., and Perez-Gil, J. (2008) Synthetic pulmonary surfactant preparations: new developments and future trends, Current medicinal chemistry 15, 393-403.

[114] Martinez-Gil, L., Perez-Gil, J., and Mingarro, I. (2008) The surfactant peptide KL4 sequence is inserted with a transmembrane orientation into the endoplasmic reticulum membrane, Biophysical journal 95, L36-38.

[115] Gonzalez-Horta, A., Andreu, D., Morrow, M. R., and Perez-Gil, J. (2008) Effects of palmitoylation on dynamics and phospholipid-bilayer-perturbing properties of the N-terminal segment of pulmonary surfactant protein SP-C as shown by 2H-NMR, Biophysical journal 95, 2308-2317.

[116] Banares-Hidalgo, A., Bolanos-Gutierrez, A., Gil, F., Cabre, E. J., Perez-Gil, J., and Estrada, P. (2008) Self-aggregation of a recombinant form of the propeptide NH2-terminal of the precursor of pulmonary surfactant protein SP-B: a conformational study, Journal of industrial microbiology & biotechnology 35, 1367-1376.

[117] Wang, L., Cruz, A., Flach, C. R., Perez-Gil, J., and Mendelsohn, R. (2007) Langmuir-Blodgett films formed by continuously varying surface pressure. Characterization by IR spectroscopy and epifluorescence microscopy, Langmuir : the ACS journal of surfaces and colloids 23, 4950-4958.

[118] Serrano, A. G., Cabre, E. J., and Perez-Gil, J. (2007) Identification of a segment in the precursor of pulmonary surfactant protein SP-B, potentially involved in pH-dependent membrane assembly of the protein, Biochimica et biophysica acta 1768, 1059-1069.

[119] Orgeig, S., Bernhard, W., Biswas, S. C., Daniels, C. B., Hall, S. B., Hetz, S. K., Lang, C. J., Maina, J. N., Panda, A. K., Perez-Gil, J., Possmayer, F., Veldhuizen, R. A., and Yan, W. (2007) The anatomy, physics, and physiology of gas exchange surfaces: is there a universal function for pulmonary surfactant in animal respiratory structures?, Integrative and comparative biology 47, 610-627.

[120] Blanco, O., and Perez-Gil, J. (2007) Biochemical and pharmacological differences between preparations of exogenous natural surfactant used to treat Respiratory Distress Syndrome: role of the different components in an efficient pulmonary surfactant, European journal of pharmacology 568, 1-15.

[121] Serrano, A. G., Ryan, M., Weaver, T. E., and Perez-Gil, J. (2006) Critical structure-function determinants within the N-terminal region of pulmonary surfactant protein SP-B, Biophysical journal 90, 238-249.

[122] Serrano, A. G., and Perez-Gil, J. (2006) Protein-lipid interactions and surface activity in the pulmonary surfactant system, Chemistry and physics of lipids 141, 105-118.

[123] Serrano, A. G., Cabre, E. J., Oviedo, J. M., Cruz, A., Gonzalez, B., Palacios, A., Estrada, P., and Perez-Gil, J. (2006) Production in Escherichia coli of a recombinant C-terminal truncated precursor of surfactant protein B (rproSP-B Delta C). Structure and interaction with lipid interfaces, Biochimica et biophysica acta 1758, 1621-1632.

[124] Ryan, M. A., Akinbi, H. T., Serrano, A. G., Perez-Gil, J., Wu, H., McCormack, F. X., and Weaver, T. E. (2006) Antimicrobial activity of native and synthetic surfactant protein B peptides, Journal of immunology 176, 416-425.

[125] Lukovic, D., Plasencia, I., Taberner, F. J., Salgado, J., Calvete, J. J., Perez-Gil, J., and Mingarro, I. (2006) Production and characterisation of recombinant forms of human pulmonary surfactant protein C (SP-C): Structure and surface activity, Biochimica et biophysica acta 1758, 509-518.

[126] Zasadzinski, J. A., Alig, T. F., Alonso, C., Bernardino de la Serna, J., Perez-Gil, J., and Taeusch, H. W. (2005) Inhibition of pulmonary surfactant adsorption by serum and the mechanisms of reversal by hydrophilic polymers: theory, Biophysical journal 89, 1621-1629.

[127] Wustneck, R., Perez-Gil, J., Wustneck, N., Cruz, A., Fainerman, V. B., and Pison, U. (2005) Interfacial properties of pulmonary surfactant layers, Advances in colloid and interface science 117, 33-58.

[128] Taeusch, H. W., Bernardino de la Serna, J., Perez-Gil, J., Alonso, C., and Zasadzinski, J. A. (2005) Inactivation of pulmonary surfactant due to serum-inhibited adsorption and reversal by hydrophilic polymers: experimental, Biophysical journal 89, 1769-1779.

[129] Serrano, A. G., Cruz, A., Rodriguez-Capote, K., Possmayer, F., and Perez-Gil, J. (2005) Intrinsic structural and functional determinants within the amino acid sequence of mature pulmonary surfactant protein SP-B, Biochemistry 44, 417-430.

[130] Ryan, M. A., Qi, X., Serrano, A. G., Ikegami, M., Perez-Gil, J., Johansson, J., and Weaver, T. E. (2005) Mapping and analysis of the lytic and fusogenic domains of surfactant protein B, Biochemistry 44, 861-872.

[131] Plasencia, I., Keough, K. M., and Perez-Gil, J. (2005) Interaction of the N-terminal segment of pulmonary surfactant protein SP-C with interfacial phospholipid films, Biochimica et biophysica acta 1713, 118-128.

[132] Plasencia, I., Rivas, L., Keough, K. M., Marsh, D., and Perez-Gil, J. (2004) The N-terminal segment of pulmonary surfactant lipopeptide SP-C has intrinsic propensity to interact with and perturb phospholipid bilayers, The Biochemical journal 377, 183-193.

[133] Cruz, A., Vazquez, L., Velez, M., and Perez-Gil, J. (2004) Effect of pulmonary surfactant protein SP-B on the micro- and nanostructure of phospholipid films, Biophysical journal 86, 308-320.

[134] Bernardino de la Serna, J., Perez-Gil, J., Simonsen, A. C., and Bagatolli, L. A. (2004) Cholesterol rules: direct observation of the coexistence of two fluid phases in native pulmonary surfactant membranes at physiological temperatures, The Journal of biological chemistry 279, 40715-40722.

[135] Wustneck, N., Wustneck, R., Perez-Gil, J., and Pison, U. (2003) Effects of oligomerization and secondary structure on the surface behavior of pulmonary surfactant proteins SP-B and SP-C, Biophysical journal 84, 1940-1949.

[136] Perez-Gil, J. (2002) Molecular interactions in pulmonary surfactant films, Biology of the neonate 81 Suppl 1, 6-15.

[137] Bi, X., Flach, C. R., Perez-Gil, J., Plasencia, I., Andreu, D., Oliveira, E., and Mendelsohn, R. (2002) Secondary structure and lipid interactions of the N-terminal segment of pulmonary surfactant SP-C in Langmuir films: IR reflection-absorption spectroscopy and surface pressure studies, Biochemistry 41, 8385-8395.

[138] Plasencia, I., Rivas, L., Casals, C., Keough, K. M., and Perez-Gil, J. (2001) Intrinsic structural differences in the N-terminal segment of pulmonary surfactant protein SP-C from different species, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 129, 129-139.

[139] Plasencia, I., Cruz, A., Lopez-Lacomba, J. L., Casals, C., and Perez-Gil, J. (2001) Selective labeling of pulmonary surfactant protein SP-C in organic solution, Analytical biochemistry 296, 49-56.

[140] Plasencia, I., Cruz, A., Casals, C., and Perez-Gil, J. (2001) Superficial disposition of the N-terminal region of the surfactant protein SP-C and the absence of specific SP-B-SP-C interactions in phospholipid bilayers, The Biochemical journal 359, 651-659.

[141] Perez-Gilabert, M., Morte, A., Honrubia, M., and Garcia-Carmona, F. (2001) Partial purification, characterization, and histochemical localization of fully latent desert truffle (Terfezia claveryi Chatin) polyphenol oxidase, Journal of agricultural and food chemistry 49, 1922-1927.

[142] Perez-Gil, J. (2001) Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics, Pediatric pathology & molecular medicine 20, 445-469.

[143] Oviedo, J. M., Valino, F., Plasencia, I., Serrano, A. G., Casals, C., and Perez-Gil, J. (2001) Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay, Analytical biochemistry 293, 78-87.

[144] Oviedo, J. M., Casals, C., and Perez-Gil, J. (2001) Pulmonary surfactant protein SP-B is significantly more immunoreactive in anionic than in zwitterionic bilayers, FEBS letters 494, 236-240.

[145] Worthman, L. A., Nag, K., Rich, N., Ruano, M. L., Casals, C., Perez-Gil, J., and Keough, K. M. (2000) Pulmonary surfactant protein A interacts with gel-like regions in monolayers of pulmonary surfactant lipid extract, Biophysical journal 79, 2657-2666.

[146] Ruano, M. L., Garcia-Verdugo, I., Miguel, E., Perez-Gil, J., and Casals, C. (2000) Self-aggregation of surfactant protein A, Biochemistry 39, 6529-6537.

[147] Cruz, A., Worthman, L. A., Serrano, A. G., Casals, C., Keough, K. M., and Perez-Gil, J. (2000) Microstructure and dynamic surface properties of surfactant protein SP-B/dipalmitoylphosphatidylcholine interfacial films spread from lipid-protein bilayers, European biophysics journal : EBJ 29, 204-213.

[148] Ruano, M. L., Nag, K., Casals, C., Perez-Gil, J., and Keough, K. M. (1999) Interactions of pulmonary surfactant protein A with phospholipid monolayers change with pH, Biophysical journal 77, 1469-1476.

[149] Ruano, M. L., Perez-Gil, J., and Casals, C. (1998) Effect of acidic pH on the structure and lipid binding properties of porcine surfactant protein A. Potential role of acidification along its exocytic pathway, The Journal of biological chemistry 273, 15183-15191.

[150] Ruano, M. L., Nag, K., Worthman, L. A., Casals, C., Perez-Gil, J., and Keough, K. M. (1998) Differential partitioning of pulmonary surfactant protein SP-A into regions of monolayers of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylcholine/dipalmitoylphosphatidylglycerol, Biophysical journal 74, 1101-1109.

[151] Perez-Gil, J., and Keough, K. M. (1998) Interfacial properties of surfactant proteins, Biochimica et biophysica acta 1408, 203-217.

[152] Nag, K., Perez-Gil, J., Ruano, M. L., Worthman, L. A., Stewart, J., Casals, C., and Keough, K. M. (1998) Phase transitions in films of lung surfactant at the air-water interface, Biophysical journal 74, 2983-2995.

[153] Cruz, A., Marsh, D., and Perez-Gil, J. (1998) Rotational dynamics of spin-labelled surfactant-associated proteins SP-B and SP-C in dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol bilayers, Biochimica et biophysica acta 1415, 125-134.

[154] Cruz, A., Casals, C., Plasencia, I., Marsh, D., and Perez-Gil, J. (1998) Depth profiles of pulmonary surfactant protein B in phosphatidylcholine bilayers, studied by fluorescence and electron spin resonance spectroscopy, Biochemistry 37, 9488-9496.

[155] Casals, C., Varela, A., Ruano, M. L., Valino, F., Perez-Gil, J., Torre, N., Jorge, E., Tendillo, F., and Castillo-Olivares, J. L. (1998) Increase of C-reactive protein and decrease of surfactant protein A in surfactant after lung transplantation, American journal of respiratory and critical care medicine 157, 43-49.

[156] Nag, K., Taneva, S. G., Perez-Gil, J., Cruz, A., and Keough, K. M. (1997) Combinations of fluorescently labeled pulmonary surfactant proteins SP-B and SP-C in phospholipid films, Biophysical journal 72, 2638-2650.

[157] Cruz, A., Casals, C., Keough, K. M., and Perez-Gil, J. (1997) Different modes of interaction of pulmonary surfactant protein SP-B in phosphatidylcholine bilayers, The Biochemical journal 327 ( Pt 1), 133-138.

[158] Ruano, M. L., Miguel, E., Perez-Gil, J., and Casals, C. (1996) Comparison of lipid aggregation and self-aggregation activities of pulmonary surfactant-associated protein A, The Biochemical journal 313 ( Pt 2), 683-689.

[159] Nag, K., Perez-Gil, J., Cruz, A., Rich, N. H., and Keough, K. M. (1996) Spontaneous formation of interfacial lipid-protein monolayers during adsorption from vesicles, Biophysical journal 71, 1356-1363.

[160] Nag, K., Perez-Gil, J., Cruz, A., and Keough, K. M. (1996) Fluorescently labeled pulmonary surfactant protein C in spread phospholipid monolayers, Biophysical journal 71, 246-256.

[161] Perez-Gil, J., Casals, C., and Marsh, D. (1995) Interactions of hydrophobic lung surfactant proteins SP-B and SP-C with dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol bilayers studied by electron spin resonance spectroscopy, Biochemistry 34, 3964-3971.

[162] Cruz, A., Casals, C., and Perez-Gil, J. (1995) Conformational flexibility of pulmonary surfactant proteins SP-B and SP-C, studied in aqueous organic solvents, Biochimica et biophysica acta 1255, 68-76.

[163] Perez-Gil, J., Lopez-Lacomba, J. L., Cruz, A., Beldarrain, A., and Casals, C. (1994) Deacylated pulmonary surfactant protein SP-C has different effects on the thermotropic behaviour of bilayers of dipalmitoylphosphatidyl-glycerol (DPPG) than the native acylated protein, Biochemical Society transactions 22, 372S.

[164] Perez-Gil, J., and Keogh, K. M. (1994) Structural similarities between myelin and hydrophobic surfactant associated proteins: protein motifs for interacting with bilayers, Journal of theoretical biology 169, 221-229.

[165] Casals, C., Ruano, M. L., Miguel, E., Sanchez, P., and Perez-Gil, J. (1994) Surfactant protein-C enhances lipid aggregation activity of surfactant protein-A, Biochemical Society transactions 22, 370S.

[166] Perez-Gil, J., Cruz, A., and Casals, C. (1993) Solubility of hydrophobic surfactant proteins in organic solvent/water mixtures. Structural studies on SP-B and SP-C in aqueous organic solvents and lipids, Biochimica et biophysica acta 1168, 261-270.

[167] Morrow, M. R., Perez-Gil, J., Simatos, G., Boland, C., Stewart, J., Absolom, D., Sarin, V., and Keough, K. M. (1993) Pulmonary surfactant-associated protein SP-B has little effect on acyl chains in dipalmitoylphosphatidylcholine dispersions, Biochemistry 32, 4397-4402.

[168] Casals, C., Miguel, E., and Perez-Gil, J. (1993) Tryptophan fluorescence study on the interaction of pulmonary surfactant protein A with phospholipid vesicles, The Biochemical journal 296 ( Pt 3), 585-593.

[169] Perez-Gilabert, M., Sanchez-Ferrer, A., and Garcia-Carmona, F. (1992) Application of active-phase plot to the kinetic analysis of lipoxygenase in reverse micelles, The Biochemical journal 288 ( Pt 3), 1011-1015.

[170] Perez-Gil, J., Tucker, J., Simatos, G., and Keough, K. M. (1992) Interfacial adsorption of simple lipid mixtures combined with hydrophobic surfactant protein from pig lung, Biochemistry and cell biology = Biochimie et biologie cellulaire 70, 332-338.

[171] Perez-Gil, J., Nag, K., Taneva, S., and Keough, K. M. (1992) Pulmonary surfactant protein SP-C causes packing rearrangements of dipalmitoylphosphatidylcholine in spread monolayers, Biophysical journal 63, 197-204.

[172] Perez-Gil, J., and Keough, K. M. (1991) Identical and similar amino acid sequences in the pulmonary surfactant proteins SP-B and SP-C and hemerythrin and myohemerythrin--an example of "biochemical Velcro"?, Biochemistry international 25, 715-721.